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primer-extension method

См. также в других словарях:

  • Primer Extension — Die Primer Extension [ˊpʁaɪ̯mɐ][ɪkˊstenʃn] ist eine molekularbiologische Methode, die im Allgemeinen zur Bestimmung der Basensequenz des 5 Endes der mRNA dient. Dazu werden aus einem Gemisch von RNA Molekülen die 5 Enden einer bestimmten RNA… …   Deutsch Wikipedia

  • Primer (molecular biology) — A primer is a strand of nucleic acid that serves as a starting point for DNA synthesis. They are required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of… …   Wikipedia

  • Overlap extension polymerase chain reaction — This page assumes familiarity with the terms and components used in polymerase chain reaction (PCR) process. The overlap extension polymerase chain reaction (or OE PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension… …   Wikipedia

  • Single Base Extension — (SBE) is a method for determining the identity of a nucleotide base at a specific position along a nucleic acid. The method is used to identify a single nucleotide polymorphism (SNP).In the method, invented by Phillip Goelet, Michael Knapp and… …   Wikipedia

  • SNP genotyping — is the measurement of genetic variations of single nucleotide polymorphisms (SNPs) between members of a species. It is a form of genotyping, which is the measurement of more general genetic variation. SNPs are one of the most common types of… …   Wikipedia

  • Bisulfite sequencing — Figure 1: Outline of bisulfite conversion of sample sequence of genomic DNA. Nucleotides in blue are unmethylated cytosines converted to uracils by bisulfite, while red nucleotides are 5 methylcytosines resistant to conversion …   Wikipedia

  • Site-directed mutagenesis — Site directed mutagenesis, also called site specific mutagenesis or oligonucleotide directed mutagenesis, is a molecular biology technique in which a mutation is created at a defined site in a DNA molecule. In general, this form of mutagenesis… …   Wikipedia

  • Метод удлинения затравки м у праймера — Метод удлинения затравки, м. у. праймера * метад падаўжэння запала, м. п. праймера * primer extension method метод получения ДНК копий на матрице мРНК …   Генетика. Энциклопедический словарь

  • DNA footprinting — is a method of investigating the sequence specificity of DNA binding proteins in vitro. This technique can be used to study protein DNA interactions both outside and within cells. The regulation of transcription has been studied extensively, and… …   Wikipedia

  • MAGIChip — MAGIChips, also known as Microarrays of Gel immobilized Compounds on a chip or Three dimensional DNA Microarrays are devices for molecular hybridization produced by immobilizing oligonucleotides, DNA, enzymes, antibodies, and other compounds on a …   Wikipedia

  • Polymerase chain reaction optimization — The polymerase chain reaction (PCR) is a commonly used molecular biology tool for amplifying DNA, and various techniques for PCR optimization have been developed by molecular biologists to improve PCR performance and minimize failure. Contents 1… …   Wikipedia

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